【摘 要】
：
In this paper, a universal effective novel method of constructing tandem genes cluster for isoflavone engineering was reported. A tandem genes cluster CHS-CHI-I
【机 构】
：
China-UK HUST-RRes Genetics Engineering and Genomics Joint Laboratory, Genetic Engineering Internati
论文部分内容阅读
In this paper, a universal effective novel method of constructing tandem genes cluster for isoflavone engineering was reported. A tandem genes cluster CHS-CHI-IFS (rIFS) of secondary metabolites of plant isoflavones was constructed by using the chalcone synthase (CHS), chalcone isomerase (CHI), and isoflavone synthase (IFS) (GenBank accession numbers EU526827,EU526829, EU526830) in only one recombination with the pET22b vector. The resulting expression vector pET-rIFS was heterogeneously expressed and co-incubated with barrenwort extractions, and the genistein-like component was detected.
其他文献
土壤盐化、高温及遮荫等逆境是限制全球小麦生产力的主要非生物因子,而利用小麦-黑麦1BL/1RS易位染色体培育的“持绿”小麦新品种的产量得到大幅度的提高。为探讨其耐盐性,本研究对含有1BL/1RS易位染色体的三个“持绿”小麦新品种：川农12(CN12),川农17(CN17)和川农18(CN18),以及用作对照且同样含有1BL/1RS易位染色体的洛夫林10(LV10)和非易位系小麦基因型绵阳11(MY
本试验于2007-2009年在扬州大学江苏省作物遗传生理实验室试验场实施,采用盆钵试验研究不同土壤速效磷含量(低磷土壤30mg/kg),不同施磷(P2O5)量(144kg/hm2、108kg/hm2、0kg/hm2)对弱筋小麦扬麦15淀粉合成关键酶基因表达、相应酶活性、淀粉积累以及加工品质的调节效应,旨从栽培生理的角度解释弱筋小麦籽粒淀粉合成过程中有关的生理现象,以期为不同速效磷含量土壤上合理施用
This study explored the effects of nuclear factor-kappa B(NF-kB)inhibitor Bay 11-7082 on Fas/FasL system and Fas-mediated apoptosis in cell line HL-60 cells.The
The autoimmune regulator(AIRE)is a crucial factor for the induction of central tolerance,and mutations in this gene lead to abnormal immune responses.However,th
Potent combination antiretroviral therapy (cART)has significantly improved the life expectancy of people living with human immunodeficiency virus(HIV),but it ha
唱金梅种地膜棉效益高卢龙县唱石门村农民唱金梅１９９４年种了一亩地膜棉，收籽棉３３０公斤，获纯效益２４００元。她的做法是：一、选优种，造足墒，确保全苗选用耐瘠薄不早衰的中棉二号优种。播前深耕（３０厘米
In order to investigate the effect of paeoniflorin (PAE)on hepatic fibrosis of mice with Schistosomiasis japonica in vivo and in vitro,a model of hepatic fibros
Modified laparoscopic microsurgical tubal anastomosis is an alteative for microsurgical anastomosis via laparotomy to reverse sterilization in women with renewe
A new region of interest (ROI) coding called partial multiply bitplane alternating shift (PMBAShift) is presented in this paper. In PMBASluft, the partial most
The aim of this paper is to develop an applicable Random Amplified Polymorphic DNAs (RAPD) method for genotyping Neisseria gonorrhoeae strain,and discuss the po