论文部分内容阅读
目的：探讨健胎液防治胎儿宫内生长迟缓的机理，应用血清药理学方法研究健胎液对缺氧人脐静脉内皮细胞（ＨＵＶＥＣ）分泌血管活性物质内皮素（ＥＴ）、前列环素（ＰＧＩ_２）、一氧化氮（ＮＯ）的影响，以及该方药对缺氧ＨＵＶＥＣ形态结构上的保护作用。方法：将ＨＵＶＥＣ随机分成四组：正常组、缺氧组、血清组、健胎液组，观察细胞形态的改变；检测内皮素（ＥＴ）、前列环素（ＰＧＩ_２）和一氧化氮（ＮＯ），进行统计学处理。结果：健胎液可显著提高缺氧ＨＵＪＶＥＣ合成分泌ＰＧＩ_２、ＮＯ（Ｐ ＜ ０．０５～０．０１），同时，ＥＴ的释放显著降低（Ｐ_４ｈ＜ ０．０５）。从超微结构上观察，健胎液能明显减轻缺氧对ＨＵＶＥＣ的细胞器和细胞核的损伤，尤以保护线粒体免受缺氧损伤的作用更显著。结论；通过增强血管内皮细胞对缺氧的耐受性，调节血管性物质的分泌是健胎液防治胎儿宫内生长迟缓的机制之一。
Objective: To investigate the mechanism of Jiantai liquid in preventing and treating fetal intrauterine growth retardation. Serum pharmacological methods were used to study the effect of Jiantai liquid on the secretion of vasoactive substances endothelin (ET) and prostacyclin (PGI_2) from hypoxia human umbilical vein endothelial cells (HUVEC). The effect of nitric oxide (NO) and the protective effect of this prescription on the morphological structure of hypoxic HUVEC. Methods: HUVECs were randomly divided into four groups: normal group, hypoxia group, serum group and Jiantai liquid group. The changes of cell morphology were observed; endothelin (ET), prostacyclin (PGI_2) and nitric oxide (NO) were detected. For statistical processing. Results: Jiantai liquid could significantly increase the synthesis and secretion of PGI 2 and NO by hypoxia HUJVEC (P < 0.05-0.01), and the release of ET was significantly decreased (P <4h <0.05). Observed from the ultrastructure, Jiantai liquid can significantly reduce the damage of hypoxia on the organelles and nuclei of HUVEC, especially to protect mitochondria from hypoxia injury. Conclusion: It is one of the mechanisms of Jiantai liquid to prevent fetal intrauterine growth retardation by enhancing the tolerance of vascular endothelial cells to hypoxia and regulating the secretion of vascular substances.