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目的:建立HPLC波长切换联合梯度洗脱法同时测定肝炎康复丸中6种主要成分[(R,S)-告依春、丹酚酸B、丹参酮ⅡA、木犀草苷、对羟基苯乙酮、滨蒿内酯]的含量.方法:色谱柱为Agilent TC-C18柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.1％磷酸溶液梯度洗脱,流速为0.9 ml·min-1,检测波长分别为245 nm[检测(R,S)-告依春]、270 nm(检测丹酚酸B和丹参酮ⅡA)、348 nm(检测木犀草苷)、278 nm(检测对羟基苯乙酮和滨蒿内酯),柱温为25℃,进样量为10μl.结果:(R,S)-告依春、丹酚酸B、丹参酮ⅡA、木犀草苷、对羟基苯乙酮、滨蒿内酯的线性范围分别为1.99～49.75μg·ml-1(r=0.9999)、18.66～466.50μg·ml-1(r=0.9994)、2.25～56.25μg·ml-1(r=0.9998)、2.62～65.50μg·ml-1(r=0.9998)、2.48～62.00μg·ml-1(r=0.9992)和2.55～63.75μg·ml-1(r=0.9996),平均加样回收率分别为98.42％、99.56％、97.96％、96.84％、98.10％和97.82％,RSD分别为0.83％、1.04％、1.53％、0.78％、1.44％和1.34％(n=6).结论:本方法简便、准确、重复性好,可为肝炎康复丸多指标定量质量评价提供依据.“,”Objective:To establish a wavelength switching HPLC with gradient elution for the simultaneous determination of six active components, [(R,S)-goitrin, salvianolic acid B, tanshinone IIA, luteolin-7-glucoside, 4-hydroxyacetophenone and scoparone ] in Ganyan Kangfu pills .Methods:An Agilent TC-C18 (250 mm ×4.6 mm, 5 μm) chromatographic column was used with the mobile phase consisting of acetonitrile and 0.1％ phosphoric acid solution with gradient elution .The flow rate was 0.9 ml· min-1 , and the column temperature was 25 ℃.( R,S)-Goitrin was detected at 245 nm, salvianolic acid B and tanshinone IIA were detected at 270 nm, luteolin-7-glucoside was detected at 348 nm, 4-hydroxyacetophenone and scoparone were detected at 278 nm.The sample volume was 10 μl.Results:The linear range of (R,S)-goitrin, salvianolic acid B, tanshinone ⅡA, luteolin-7-glucoside, 4-hydroxyacetophe-none and scoparone was 1.99-49.75 μg· ml-1(r=0.9999), 18.66-466.50 μg· ml-1(r=0.9994), 2.25-56.25 μg· ml-1(r=0.9998), 2.62-65.50 μg· ml-1(r=0.9998), 2.48-62.00 μg· ml-1(r=0.9992) and 2.55-63.75μg· ml-1(r=0.9996), respectively.The average recovery was 98.42％(RSD=0.83％), 99.56％(RSD=1.04％), 97.96％(RSD=1.53％), 96.84％(RSD=0.78％), 98.10％(RSD=1.44％) and 97.82％(RSD=1.34％)(n=6), respectively.Conclusion: The method with good reproducibility is simple and accurate , which provides a new way for the quality evaluation of Ganyan Kangfu pills .