论文部分内容阅读
目的 探讨机械通气对新生儿血浆中抗氧化酶 (antioxidant enzyme,AOE)活性的影响。 方法 用酶活性测定法检测 72例新生儿机械通气前后血浆中过氧化氢酶 (catalase,CAT)、谷胱甘肽过氧化物酶 (glutathione peroxidage,GPX)和超氧化物歧化酶 (superoxide dism utase,SOD)的活性。 结果 (1)机械通气前 ,早产儿与足月儿 ,低出生体重儿与正常出生体重儿血浆 GPX分别为(90± 6 1)、(116± 5 1)、(94± 6 1)、(113± 5 2 ) k U/ L,CAT分别为 (6 8± 43)、(82± 43)、(6 9± 41)、(84±43) k U/ L,差异无显著性 (P>0 .0 5 ) ,而 SOD在早产儿和低出生体重儿较低 [分别为 (78± 11)、(84±11) k U/ L(P<0 .0 5 )。(2 )机械通气后 ,各组 GPX、CAT、SOD均随通气时间的延长而下降 ,但足月儿、正常体重儿下降幅度较小 ,而早产儿、低出生体重儿下降幅度较大。通气后 3d GPX、CAT、SOD在早产儿分别为 (72± 47)、(5 1± 33)、(5 3± 9) ,足月儿分别为 (10 2± 49)、(78± 37)、(87± 11) ,低出生体重儿分别为 (70± 48)、(4 9± 31)、(5 1± 9) ,正常出生体重儿分别为 (10 3± 47)、(74± 2 3)、(85± 12 ) k U/L,早产儿低于足月儿 ,低出生体重儿各项均低于正常出生体重儿 (P<0 .0 1)。 结论 机械通气可降低新生
Objective To investigate the effect of mechanical ventilation on the activity of antioxidant enzyme (AOE) in neonatal plasma. Methods Serum levels of catalase (CAT), glutathione peroxidase (GPX) and superoxide dismutase (TNF-α) were measured in 72 neonates before and after mechanical ventilation by enzymatic activity assay , SOD) activity. Results (1) The GPX of premature infants, term infants, low birth weight infants and normal birth weight infants before mechanical ventilation were (90 ± 6 1), (116 ± 5 1), (94 ± 6 1), 113 ± 52, k U / L and CAT were (68 ± 43), (82 ± 43), (69 ± 41) and (84 ± 43) kU / L, respectively. 0.05), while SOD was lower in preterm and low birth weight infants (78 ± 11 and 84 ± 11 kU / L, respectively, P <0.05). (2) After mechanical ventilation, the GPX, CAT and SOD in each group decreased with the prolongation of ventilation time. However, the decrease in full-term infants and normal weight infants was smaller, while that of premature infants and low birth weight infants was significantly decreased. The levels of GPX, CAT and SOD in preterm infants were (72 ± 47), (51 ± 33) and (53 ± 9) days, respectively, and full-term infants were (102 ± 49) and (78 ± 37) , (87 ± 11) and (70 ± 48), (49 ± 31) and (51 ± 9), respectively. The normal birth weight children were (103 ± 47) and 3) and (85 ± 12) kU / L respectively. The preterm infants were lower than the full-term infants, and those with low birth weight were lower than those with normal birth weight (P <0.01). Conclusion Mechanical ventilation can reduce newborn