论文部分内容阅读
云南烟草丛枝症病害四环素治疗无效，可经汁液摩擦和蚜虫传染，病程表现为潜伏期、过敏反应期、缩顶期和丛枝期等４个阶段，细胞内产生质膜体和细胞质泡囊等膜系统畸形病变，病株总核酸中存在特殊的超稳定低分子量ＲＮＡ，ＮｅｓｔｅｄＰＣＲ在病株总核酸中（引物：Ｒ１６ｍＲ１／Ｒ１６ｍＦ２，Ｒ１６Ｒ２／Ｒ１６Ｆ２）未检测到植原体１６ｓＲＮＡ基因．说明云南烟草丛枝症病害和烟草丛枝病是不同的病害，病原不是植原体，该病表现出病毒侵染的特征，可能由病毒或类似于病毒的病原引起．依据病害的症状和侵染特征，建议将“云南烟草丛枝症病害”称呼为“烟草簇矮病（Ｔｏｂａｃｃｏｂｕｓｈｙｄｗａｒｆ）”．
Tobacco witches bush disease Yunnan tetracycline treatment ineffective, can be juice friction and aphids infection, the course of the disease showed latent period, allergic reaction period, the shrinkage of the top and the branches of the four stages, the plasma membrane and cytoplasmic vesicles and other cells Membrane system deformity lesions, the total nucleic acid in the plant there is a special ultra-stable low molecular weight RNA, Nested PCR in the total plant nucleic acid (primer: R16mR1 / R16mF2, R16R2 / R16F2) did not detect the 16sRNA gene of phytoplasma. The results showed that the tobacco bud witches disease and tobacco branching disease were different diseases in Yunnan and the pathogen was not phytoplasma. The disease showed the characteristic of virus infection, which may be caused by virus or virus-like pathogen. According to the symptom of the disease and the characteristics of the infection, it is suggested that “Yunnan Tobacco Bush witches disease” be called “Tobaccobushydwarf”.