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目的通过对婴幼儿感染性疾病中微量元素的检测,了解这些微量元素之间的变化与感染性疾病之间的关系,为疾病的预防和治疗提供依据。方法对120例患儿末梢全血40微升两份,一份血样放入锌、铁、钙试剂,静置20分钟,经半径为5厘米的离心机离心(2500r/min),取上清液由专业检验员采用微分电位溶出法测定锌、铁、钙、镁含量水平;另一份血样加入铅铜试剂,静置5分钟,采用极谱分析法测定铅铜含量水平。结果实验组婴幼儿锌、铁、钙含量低于健康对照组P<0.01,差异有非常显著性;铅高于对照组P<0.01,差异有非常显著性;铜镁与对照组差异无显著性,P>0.05。结论感染性疾病的患儿血锌、铁、钙低于健康儿,血铅高于健康儿。
Objective To detect the trace elements in infectious diseases of infants and young children, to understand the relationship between the changes of these trace elements and infectious diseases, and to provide basis for disease prevention and treatment. Methods One hundred and twenty children were given 40 microliter aliquots of whole blood. One blood sample was placed in zinc, iron and calcium reagent and allowed to stand for 20 minutes. Centrifuged with a 5 cm radius centrifuge (2500 r / min) The liquid zinc, iron, calcium and magnesium levels were determined by professional examiners using the differential potentiometric stripping method. Another sample of lead and copper was added to the blood sample and allowed to stand for 5 minutes. The level of lead and copper was determined by polarographic analysis. Results The contents of zinc, iron and calcium in infants in the experimental group were lower than those in the healthy control group (P <0.01), the difference was significant (P <0.01), the difference was significant (P <0.01) , P> 0.05. Conclusion Infectious diseases in children with zinc, iron, calcium than healthy children, blood lead higher than healthy children.