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从7月27日至9月15日,我们从新梢上休眠的腋芽中的主芽上分离出带有2—4个叶原基的芽尖。采芽的品种上带有卷叶病毒病。将这些芽尖放置在MS—Nitsch培养基中(含BA 2毫克/立升),经过6个月的休眠,芽尖从二月份开始增殖。从芽尖长出的新梢上形成的芽眼数在6月份达到最高峰。往后芽的出现逐渐减少。其中黑彼诺有12％的芽和Merlot有20％的芽能发育成为新梢,最初的植株具有大量叶片。将BA的浓度降低到0.2毫克/立升时,可以促使新梢长根。长根的新梢移植到珍珠
From July 27 to September 15, we isolated shoot tips with 2-4 leaf primordia from the main shoots in axillary buds dormant on shoots. Budding varieties with leaf-roll virus disease. These bud tips were placed in MS-Nitsch medium (BA 2 mg / liter), and buds began to proliferate from February after 6 months of dormancy. The number of buds formed on the shoots emerging from the bud tips reached its peak in June. The emergence of buds gradually reduced. Among them, Pinot Grigio has 12% buds and Merlot has 20% buds that develop into new shoots. The initial plant has a large number of leaves. When the concentration of BA is reduced to 0.2 mg / litter, the root of the shoot can be induced. The long shoots are transplanted to the pearl