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利用6×3析因试验设计,设置6组pH(5.0、6.0、7.0、8.0、9.0和10.0)和3组体质量(1.02、5.13和10.31g),在试验室条件下研究了酸碱胁迫对不同体质量尼罗罗非鱼幼鱼的呼吸、排泄、乳酸脱氢酶(LDH)和Na+-K+ATPase活力的影响.结果表明:尼罗罗非鱼幼鱼的耗氧率和排氨率随pH值的升高先上升后下降,在pH7.0～8.0时达到最高值.耗氧量与排氨量随体质量的增加而增加并呈幂函数相关.体质量的一次和pH的一次、二次效应对尼罗罗非鱼幼鱼的耗氧率和排氨率有极显著的影响(P<0.01);体质量与pH之间无互作效应(P>0.05);建立了pH与体质量对耗氧率、排氨率之间的回归方程,决定系数分别达到0.942和0.936;体质量对氧氮比(O∶N)值有显著影响(P<0.01),酸碱胁迫使尼罗罗非鱼幼鱼的能源物质利用模式发生改变.高pH条件不利于LDH活力的增加,但有利于Na+-K+ATPase活力的上升,pH的一次和二次效应对酶活有极显著影响(P<0.01),体质量对Na+-K+ATPase活力没有显著影响,且pH与体质量对酶活无互作效应(P>0.05).
Using 6 × 3 factorial design, six pH values (5.0, 6.0, 7.0, 8.0, 9.0 and 10.0) and three body weights (1.02, 5.13 and 10.31g) were set up to study the effects of acid-base stress On the respiration, excretion, activities of lactate dehydrogenase (LDH) and Na + -K + ATPase in juvenile Nile tilapia (Nile tilapia) with different body weight.The results showed that: The rate increased first and then decreased with the increase of pH value, reaching the highest value at pH 7.0 ~ 8.0. The oxygen consumption and the amount of ammonia excretion increased with the increase of body mass and showed a power function correlation. The primary and secondary effects had a very significant effect on the oxygen consumption rate and ammonia excretion rate of juvenile Nile tilapia (P <0.01). There was no interaction between body weight and pH (P> 0.05) The regression equation between pH and body mass ratio of oxygen consumption and ammonia excretion was 0.942 and 0.936, respectively. The body mass had a significant effect on O / N ratio (P <0.01), acid-base stress Which changed the energy utilization pattern of juvenile Nile tilapia.The high pH condition is not conducive to the increase of LDH activity, but is conducive to the increase of Na + -K + ATPase activity, the primary and secondary effects of pH on the enzyme activity Significantly Effect (P <0.01), body weight had no significant effect on the Na + -K + ATPase activity, and pH on the enzyme activity and body mass without interaction effects (P> 0.05).