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目的为了解通辽市科左中旗健康人群脑膜炎奈瑟氏菌带菌情况及其免疫抗体水平。方法采集调查对象咽拭子,现场接种至羊血巧克力琼脂培养基,于5%的二氧化碳环境37℃培养24～72h,进行脑膜炎奈瑟菌分离和鉴定;血清抗体测定采用酶联免疫吸附试验法。结果 221名健康人中,检出革兰氏染色阴性脑膜炎双球菌13株,总带菌率为3.81%。A群抗体阳性率为71.91%,C群抗体阳性率为47.51%。结论本地区流脑抗体水平较低,没有达到免疫屏障的作用,要加强流脑防控工作,提高人群免疫力。为了解通辽市科左中旗健康人群脑膜炎奈瑟氏菌(Nm)带菌情况及其免疫抗体水平,于2007年10月对我市科左中旗保康镇健康人群进行脑膜炎奈瑟菌带菌情况及其免疫抗体水平监测,为今后流脑预防与控制提供科学依据。
Objective To understand the status of Neisseria meningitidis infection and immune antibody levels among healthy population in Kezuozhongqi, Tongliao City. Methods Throat swabs were collected and inoculated into sheep blood chocolate agar medium and cultured in a 5% carbon dioxide atmosphere at 37 ℃ for 24-72h. Neisseria meningitidis was isolated and identified. Serum antibody was determined by enzyme linked immunosorbent assay law. Results Among 221 healthy people, 13 Gram-negative meningococcal strains were detected with a total carrier rate of 3.81%. The positive rate of antibody of group A was 71.91%, the positive rate of antibody of group C was 47.51%. Conclusion The meningococcal antibody in this area is low and does not reach the immune barrier. It is necessary to strengthen the prevention and control of meningitis and improve the immunity of the population. To understand the status of Neisseria meningitidis (Nm) -containing and immunized antibody in healthy population of Kezuozhong Banner in Tongliao City, Neisseria meningitidis Carrying bacteria and immune antibody level monitoring for the future prevention and control of the flow of the brain to provide a scientific basis.