目的探讨载脂蛋白J(ApoJ)外显子3、4、7、8基因多态性与2型糖尿病(T2DM)的关系。方法2002-11~2003-06对贵阳医学院附属医院,利用聚合酶链式反应-变性梯度凝胶电泳技术(PCR-DGGE)对61例T2DM患者、60例正常对照者的ApoJ外显子3、4、7、8基因进行基因筛查,并对异常条带进行测序分析。结果我国ApoJ基因外显子7缺失与插入(DVSI)基因型在T2DM与正常对照组间分布差异显著(P<0.01),ApoJ外显子3、4、8差异无显著性(P>0.05)。结论我国2型糖尿病患者ApoJ外显子7基因插入与缺失多态性可能与2型糖尿病发病有关联,是2型糖尿病的危险因素之一。 Objective To investigate the relationship between polymorphisms of 3,4,7,8 exon 3, 7 and apolipoprotein J (ApoJ) and type 2 diabetes mellitus (T2DM). Methods From June 2002 to June 2003, 61 cases of T2DM and 60 cases of normal control were enrolled in this study. The ApoJ exon 3 , 4,7,8 gene for gene screening, and abnormal bands for sequencing analysis. Results The distribution of exon 7 deletion and insertion in ApoJ gene was significantly different between T2DM and normal controls (P <0.01), but there was no significant difference in ApoJ exon 3, 4 and 8 (P> 0.05) . Conclusion ApoJ exon 7 gene insertion and deletion polymorphisms in type 2 diabetes mellitus may be associated with the development of type 2 diabetes mellitus, which is one of the risk factors for type 2 diabetes mellitus.