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本文研究了家兔下丘脑—垂体—(眼前房)卵巢轴的激素调节特点。实验用成年雌兔,在摘除两侧卵巢后立即将一片卵巢组织移植于眼前房。分别用放射免疫法及竞争性蛋白结合法测定正常对照组兔与移植组兔静脉血中FSH、LH、cAMP 及孕酮含量。结果表明,移植兔与正常兔一样,静脉注射GnRH(5微克/公斤)后30、60及240分钟,血中FSH 与LH 含量有逐渐增加趋势并显著高于基础水平。与雄兔交配后30、60、120及240分钟内,血中FSH、LH 及cAMP 的含量也明显上升,两组动物三项指标变化的幅度相似。交配,静脉注射醋酸铜、HCG 或GnRH,均可使移植卵巢发生典型点状出血,称为排卵反应。在出现排卵反应后的第二、第四与第六天,血中孕酮含量较给药前增加5—6倍。上述排卵反应,在不同程度上可被避孕一号与复方18甲基炔诺酮所抑制。证明了移植动物的下丘脑—垂体—(眼前房)卵巢轴的激素调节与正常物动相比,是基本一致的。通过透明的角膜(橱窗)可长期直接观察排卵进程的变化,为研究下丘脑—垂体—卵巢轴活动规律与筛选抗排卵药物,提供简便的慢性动物模型。
This article studies the hypothalamus - pituitary - (anterior chamber) ovarian axis of the hormone regulation characteristics. Experimental adult female rabbits, ovaries in the removal of both sides immediately after an ovarian tissue transplanted in the anterior chamber. The contents of FSH, LH, cAMP and progesterone in venous blood of rabbits in normal control group and rabbits in transplantation group were determined by radioimmunoassay and competitive protein-binding assay respectively. The results showed that the levels of FSH and LH in blood were gradually increased at the 30th, 60th and 240th minute after intravenous injection of GnRH (5μg / kg) in normal rabbits, which was significantly higher than that of normal rabbits. Within 30, 60, 120 and 240 minutes after mating with the male rabbits, the levels of FSH, LH and cAMP in the blood also increased obviously, and the amplitude of the three indexes of the two groups of animals changed similarly. Mating, intravenous injection of copper acetate, HCG or GnRH, can make a typical transplanted ovary punctate bleeding, known as ovulation reaction. In the second, fourth and sixth days after ovulation reaction, progesterone in blood increased by 5-6 times compared with that before administration. The ovulation response, to varying degrees, can be contraceptives and compound 18 norgestrel-inhibited. Proved that transplanted animal hypothalamus - pituitary - (anterior chamber) of the ovarian axis of hormonal regulation compared with the normal activity is basically the same. Through the transparent cornea (window) can be long-term direct observation of changes in ovulation process, in order to study the hypothalamic-pituitary-ovarian axis activity rules and screening anti-ovulation drugs, to provide a simple and chronic animal model.