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目的利用差相富集-免疫荧光原位杂交技术(subtraction enrichment and immunostainingfluorescence in situ hybridization,SE-iFISH)检测膀胱癌患者外周血循环肿瘤细胞(circulating tumor cells,CTCs),并探究该检测结果与肿瘤临床特征的关系。方法收集2016年4~6月收治入院的16例膀胱癌患者(实验组)临床病理资料,其中男14例,女2例,年龄(66.88±8.97)岁。另取10例非肿瘤患者为对照(对照组),其中男7例,女3例,年龄(61.90±9.13)岁。取外周静脉血各6mL,差相富集法收集外周血CTCs,肿瘤细胞标志物-iFISH检测6mL血中所有CTCs的数量及相关生物学特性,比较CTCs的数量与膀胱癌临床特征(有无血尿、肿瘤大小、数目、是否侵及肌层、原发肿瘤分期、淋巴结转移、肿瘤级别、肿瘤病理学类型等)的关系。结果实验组CTCs阳性检出率为43.75%(7/16),对照组CTCs阳性检出率为0%(0/10),两组阳性检出率差异有统计学意义(P=0.022 7)。实验组CTCs含量为0~29个,中位数为0个,对照组CTCs含量为0个,两组CTCs含量差异有统计学意义(P=0.028 3)。实验组中,有无血尿、肿瘤大小、数目、是否侵及肌层、原发肿瘤分期、淋巴结转移、肿瘤级别、肿瘤病理学类型等亚组之间,CTCs阳性检出率的差异无统计学意义。结论 SE-iFISH检测膀胱癌外周血CTCs可行性好。关于CTCs含量与膀胱癌患者临床特征的关系尚不肯定,需更大样本量予以说明。
Objective To detect circulating tumor cells (CTCs) in peripheral blood of patients with bladder cancer using subtraction enrichment and immunostaining fluorescence in situ hybridization (SE-iFISH) Characteristics of the relationship. Methods The clinical data of 16 patients with bladder cancer (experimental group) admitted to hospital from April to June in 2016 were collected, including 14 males and 2 females, with a mean age of 66.88 ± 8.97 years. Another 10 cases of non-tumor patients as a control (control group), including 7 males and 3 females, age (61.90 ± 9.13) years. Peripheral venous blood samples were collected from 6 mL of peripheral venous blood and peripheral blood CTCs were collected by differential phase enrichment. The number of all CTCs in 6 mL of blood was detected by iFISH. The number of CTCs was compared with the clinical features of bladder cancer , Tumor size, number, invasion of muscularis, primary tumor staging, lymph node metastasis, tumor grade, tumor pathology type, etc.). Results The positive rate of CTCs in the experimental group was 43.75% (7/16), while the positive rate of CTCs in the control group was 0% (0/10). There was significant difference between the two groups (P = 0.022 7) . There were 0 to 29 CTCs in the experimental group, with a median of 0, and 0 in the control group. There was significant difference in CTCs between the two groups (P = 0.0283). There was no significant difference in the positive detection rates of CTCs among the subgroups in the experimental group with or without hematuria, tumor size, number, invasion of the muscular layer, primary tumor staging, lymph node metastasis, tumor grade, tumor pathology type significance. Conclusion SE-iFISH CTCs in bladder cancer detection of the feasibility of good. The relationship between CTCs and clinical features in patients with bladder cancer is uncertain, and a larger sample size is needed.