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研究不同室温对小鼠过敏性哮喘模型脾脏、外周血细胞共刺激分子的表达及肺组织损伤的影响,探究温度与过敏性疾病严重程度的相关性。将40只昆明鼠随机分组后分别置于20±2℃(低温组)和30±2℃(高温组)饲养,每个温度组分别设置造模组和对照组。造模组小鼠分别于第1天及第8天腹腔注射致敏液0.5ml(含卵清蛋白1 mg和氢氧化铝凝胶4mg),第15天起,每天以1%卵清蛋白溶液喷雾30min,连续7d,末次喷雾18h后处死小鼠。对照组用相同体积的生理盐水处理。免疫荧光及流式细胞术检测脾脏和外周血PBMC共刺激分子的表达,ELISA检测血液中IgE、IL-4及IFN-γ的含量,取肺组织经HE染色后作组织形态学分析。造模组脾脏和外周血PBMC共刺激分子的表达均升高(P<0.05),且低温组明显高于高温组(P<0.05)。造模组血清IgE及IL-4的水平明显升高(P<0.05),低温组明显高于高温组(P<0.05);但IFN-γ的水平明显下降(P<0.05),两温度组无明显差别(P>0.05)。组织形态学的观察结果显示,造模组小鼠的支气管及肺泡腔内出现炎性细胞浸润、支气管管壁增生及肺泡黏膜肿胀水肿,且低温组比高温组严重。哮喘引发的免疫应答及肺部出现的病理改变与温度呈负相关。
To study the effect of different room temperature on the expression of costimulatory molecules in spleen and peripheral blood cells of mice with allergic asthma model and lung injury, and to explore the correlation between temperature and the severity of allergic diseases. Forty Kunming mice were randomly divided into two groups: 20 ± 2 ℃ (low temperature group) and 30 ± 2 ℃ (high temperature group), respectively. Each temperature group was given model control group and control group. Mice in model group were injected intraperitoneally with 0.5ml of sensitizing solution (containing 1 mg ovalbumin and 4 mg aluminum hydroxide gel) on day 1 and day 8, respectively. On the 15th day, the mice were treated with 1% ovalbumin solution Spray 30min, continuous 7d, the mice were sacrificed 18h after the last spray. The control group was treated with the same volume of saline. Immunofluorescence and flow cytometry were used to detect the expression of costimulatory molecules in PBMC of spleen and peripheral blood. The levels of IgE, IL-4 and IFN-γ in blood were measured by ELISA. Histopathological changes of lung tissue were analyzed by HE staining. The expression of costimulatory molecules in spleen and peripheral blood in model group were significantly higher than those in high temperature group (P <0.05). The levels of serum IgE and IL-4 in model group were significantly higher than those in high temperature group (P <0.05), but the level of IFN-γ was significantly decreased (P <0.05) No significant difference (P> 0.05). The results of histomorphology showed that inflammatory cell infiltration, bronchial wall proliferation and alveolar mucosal swelling and edema occurred in the bronchial and alveolar cavities in the model group mice, and the hypothermia group was more severe than the hyperthermia group. Immune response to asthma and pathological changes in the lungs were negatively correlated with temperature.