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本研究对妊娠三个月内的胚泡植入部位蜕膜及胎盘绒毛组织的滋养层细胞进行了光镜HE、纤维素及免疫细胞化学染色观察.光镜下,植入部位的蜕膜中弥漫地浸润着一种较大的圆形、多边形或梭形的细胞,它们核的大小、形状不一,多为单核,少数为双核或多核,胞浆丰富,嗜伊红或双色,常分布在螺旋动脉周围,浸润其管璧并侵入其管腔,甚至可完全取代其管壁。免疫细胞化学观察显示这类细胞HCG及HPL染色均可为阳性,但HCG阳性率很低,并且其HCG染色明显弱于合体滋养层细胞,HPL染色显著强于合体滋养层细胞,细胞滋养层细胞的HCG、HPL染色均为阴性。β-HCG及SP-1的染色在这几种滋养层细胞中的情况与HCG一致,而PAPP-A染色无特异性。胚泡植入部位具有上述特点的细胞即为中间型滋养层细胞,现多认为这类细胞由细胞滋养层细胞演变而来,又可演变为合体滋养层细胞,是后两种滋养层细胞的移行过渡形式。
In the present study, trophoblast cells in the decidua and placental villus tissues at blastocyst implantation sites within 3 months of pregnancy were observed under light microscope (HE), cellulose and immunocytochemical staining.Under light microscope, the decidua Diffuse infiltration of a larger round, polygonal or fusiform cells, their nuclear size, shape, mostly mononuclear, a small number of dual or multi-nuclear, abundant cytoplasm, eosinophil or bicolor, often Spread around the spiral arteries, infiltration of its pipe wall and invade its lumen, and can even completely replace its wall. Immunocytochemistry showed that these cells were positive for HCG and HPL staining, but the positive rate of HCG was low, and HCG staining was significantly weaker than that of syncytiotrophoblast cells. HPL staining was significantly stronger than that of syncytiotrophoblast, cytotrophoblast HCG, HPL staining were negative. Staining of β-HCG and SP-1 was consistent with HCG in these trophoblast cells, whereas PAPP-A staining was nonspecific. Blastocyst implantation site with the above characteristics of the cells that are intermediate trophoblast cells, are now that such cells evolved from the cells of trophoblast cells, but also evolved into syncytiotrophoblast cells, the latter two trophoblast cells Transitional form of migration.