采用经四氢呋喃和氯仿处理过的一米长的不锈钢柱,填充剂为 Bondapak C18/Corasil。流动相用二组不同极性的混合溶剂组成。A 组极性较高,由无水甲醇50毫升,加水750毫升,再加入200毫升0.1MpH4.5的磷酸钾缓冲液相混而成;极性较低的 B 组则由无水甲醇400毫升,乙腈200毫升和水200毫升的混合液中,再加入200毫升0.1M pH4.5的磷酸盐缓冲液相混而成。 A one meter long stainless steel column treated with tetrahydrofuran and chloroform was used as the filler, Bondapak C18 / Corasil. The mobile phase consisted of two sets of mixed solvents of different polarities. A group of high polarity, from 50 ml of anhydrous methanol, add water 750 ml, then add 200 ml of 0.1MpH4.5 of potassium phosphate buffer mixed; less polar group B from anhydrous methanol 400 ml , 200 ml of acetonitrile and 200 ml of water, and then 200 ml of 0.1 M phosphate buffer of pH 4.5 was mixed.