寄生真菌对根结线虫的致病力评价

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用南方根结线虫卵作接种试验,证明从根结线虫成虫及卵上分离76个菌株中大部分菌株有寄生能力,种间及株间寄生率差异明显,表明这种方法是检验寄生真菌寄生能力的一种良好方法。通过测定,76个菌株中有10个菌株对南方根结线虫卵的寄生率超过50%,种间及菌株间对卵的寄生能力存在很大差异;Paecilomyceslilacinus,Verticiliumchlamydosporium及V.lamelicola对Meloidogyneincognita,M.arenaria和M.javanica卵的寄生能力进行比较,在3种线虫之间没有明显差异,说明这3种真菌对这3种根结线虫有进行防治的可能性。首次研究了寄生真菌培养滤液对根结线虫的作用,发现大多数寄生真菌的代谢产物对幼虫有毒害作用。28个菌株中24个的培养滤液对南方根结线虫幼虫有很高的毒性作用,在4h内56%~100%幼虫不活动;P.lilacinus菌株54培养滤液对幼虫作用的时间不同,效果也有很大差异,作用4h,幼虫不活动率为100%,作用48h,幼虫死亡率为100%。在盆栽试验中,证明某些真菌对无菌土栽培的番茄上南方根结线虫有明显的防治效果。其中P.lilacinus? Inoculation experiments with Southern root-knot nematode eggs demonstrated that most of the 76 strains isolated from adult and egg of root-knot nematodes had parasitism, with significant differences among species and strains, indicating that this method is to test parasitic fungi A good way to parasitize. According to the results, 10 out of 76 strains of Paramyxomyces lilacinus, Verticiliumchlamydosporium and V. parasitica had more than 50% infestation on M. elegans eggs. lamelicola to Meloidogyneincognita, M. arenaria and M. javanica eggs parasitism ability comparison, there is no significant difference between the three kinds of nematodes, indicating that the three kinds of fungi of these three kinds of root knot nematode have the possibility of prevention and treatment. The effect of parasitic fungal culture filtrate on root-knot nematodes was first studied and it was found that the metabolites of most parasitic fungi have a toxic effect on larvae. Twenty-four of the 28 bacterial strains had a high toxic effect on root-knot nematode larvae, with 56% -100% of the larvae inactive within 4 h. Lilacinus strain 54 culture filtrate on the larvae of different time, the effect is also very different, the role of 4h, the larvae inactivation rate of 100%, 48h, the larval mortality was 100%. In the pot experiment, it was proved that some fungi had significant control effects on the root-knot nematodes on tomato cultivated in aseptic soil. Where P. lilacinus?
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