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扩展蛋白(expansins)是植物细胞壁的重要组成部分,能够引起细胞壁组分间松驰和细胞壁柔韧性增加,在植物的许多生长发育过程中发挥作用。为了揭示黄瓜扩展蛋白基因家族的功能,本研究利用全基因组测序数据鉴定黄瓜扩展蛋白基因家族,分析其结构特征及系统发育关系。黄瓜基因组测序数据分析显示,黄瓜扩展蛋白基因家族包含35个成员,包括A(21)、B(3)、LA(9)和LB(2)4个亚家族,分布在黄瓜7条染色体上。扩展蛋白氨基酸长度范围为206-295,编码蛋白质具有保守的结构域,蛋白质亚细胞定位预测结果表明所有蛋白均定位于细胞外。基因结构分析表明,黄瓜扩展蛋白四个亚家族的平均内含子数目分别为A类1.8个,B类2.7个,LA类3.8个和LB类3.5个。基因表达分析发现,该家族基因在黄瓜雌雄花蕾及果实发育过程中表达丰度较高。本研究分析了黄瓜扩展蛋白基因家族的基本信息,可为深入研究其扩展蛋白基因的分子进化与生物学功能奠定基础。
Expansins, an important part of plant cell walls, can cause relaxation of cell wall components and increased cell wall flexibility, and play a role in many plant growth and development. In order to reveal the function of the cucumber expansion protein gene family, we used genome-wide sequencing data to identify the cucumber expansion protein gene family and analyzed its structural characteristics and phylogenetic relationships. The cucumber genome sequencing data analysis showed that the cucumber expansion protein gene family contains 35 members, including A (21), B (3), LA (9) and LB (2) 4 subfamilies, distributed in seven chromosomes of cucumber. The amino acids of extended proteins range in length from 206 to 295 and encode conserved domains. The protein subcellular localization predicts that all proteins are located extracellularly. Gene structure analysis showed that the average intron numbers of four subfamilies of cucumber expansion protein were 1.8 in category A, 2.7 in category B, 3.8 in category LA and 3.5 in category LB. Gene expression analysis found that the family of genes in the cucumber male and female flower buds and fruit development in the higher abundance. In this study, we analyzed the basic information of the cucumber epicate gene family and laid the foundation for further study on the molecular evolution and biological functions of the expansin gene.