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用SRBC经不同途径(sc+ip、sc+iv、ip、iv)免疫单只动物即可诱导产生迟发型变态反应(DTH),又能同时诱导产生抗体,皮下(sc)免疫只能诱导产生DTH。这个动物模型的灵敏性用免疫抑制药物坏磷酰胺和免疫增强药物厌氧棒状杆菌菌苗进行检测。给予小鼠环磷酰胺可明显抑制DTH和抗体(IgM PFC)的反应,而厌氧棒状杆菌菌苗可明显增强小鼠的抗体反应。多项免疫功能检测与单项免疫功能检测方法所得结果非常相似,单个动物多项免疫功能检测是一个经济、省时、灵敏的评价化合物和药物的免疫毒性和药理毒性的方法。
Immunization of a single animal with SRBC via different routes (sc + ip, sc + iv, ip, iv) induces delayed-type hypersensitivity (DTH) as well as antibody production at the same time, with subcutaneous (sc) immunity induced DTH. The sensitivity of this animal model is tested with the immunosuppressive drug desmophosphamide and the immunopotentiating agent Anaerobic Corynebacter. Administration of cyclophosphamide in mice significantly inhibited the reaction of DTH and antibody (IgM PFC), whereas anaerobic coryneform bacilli vaccine significantly increased the antibody response in mice. A number of immune function tests are very similar to the results of single immunofection tests. The detection of multiple immunological functions in a single animal is an economical, time-saving and sensitive method to evaluate the immunotoxicity and pharmacological toxicity of compounds and drugs.