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目的 :观察醒脑静注射液 (XNJI)对脑缺血 再灌注损伤 (CIRI)家兔血清白介素 8(IL 8)水平的影响 ,并探讨其脑保护机制。方法 :2 8只家兔随机分为假手术对照组 (C组 )、缺血再灌注组 (I R组 )和醒脑静注射液组 (XNJI组 ) ,分别在缺血前、缺血 3 0min、再灌注 3 0min、60min、12 0min时取静脉血 ,测定血清IL 8浓度 ,实验结束后取脑组织观察脑超微结构的变化。结果 :脑缺血 再灌注期间 ,I R组不同时点血清IL 8水平明显高于XNJI组 ,缺血 3 0min为 (0 .62± 0 .18)ng·L-1及 (0 .43± 0 .0 8)ng·L-1,P <0 .0 5 ;再灌注 3 0min ,为血清IL 8水平 (0 .73± 0 .19)ng·L-1及 (0 .45± 0 .0 9)ng·L-1;再灌注 60min ,血清IL 8水平为 (0 .87± 0 .2 9)ng·L-1及 (0 .47± 0 .0 6)ng·L-1;再灌注 12 0min血清IL 8水平为 (1.0 3± 0 .43 )ng·L-1及 (0 .44± 0 .0 7)ng·L-1,再灌注各时点组均P <0 .0 1,超微结构发生异常改变 ;XNJI组不同时点血清IL 8浓度显著低于I R组 (XNJI组缺血 3 0min为(0 .48± 0 .0 7)ng·L-1,P <0 .0 5 ;再灌注 3 0min为 (0 .5 0± 0 .0 8)ng·L-1;再灌注 60min为 (0 .5 5± 0 .14)ng·L-1;再灌注 12 0min为 (0 .5 9± 0 .17)ng·L-1,再灌注各时点组P <0 .0 1) ,其超微结构异常改变较I R组?
Objective: To observe the effect of xingnaojing injection (XNJI) on the level of serum interleukin 8 (IL-8) in rabbits with cerebral ischemia-reperfusion injury (CIRI), and to explore its mechanism of brain protection. Methods: Twenty-eight rabbits were randomly divided into sham-operation control group (C group), ischemia-reperfusion group (IR group) and xingnaojing injection group (XNJI group) , Reperfusion 3 0min, 60min, 120min when venous blood was taken, serum IL 8 concentration was measured after the experiment, brain tissue was taken to observe the ultrastructure changes. Results: The level of IL-8 in IR group was significantly higher than that in XNJI group at 30 min after ischemia / reperfusion (0 .62 ± 0.18 ng · L-1 and (0.43 ± 0) (0. 73 ± 0.19) ng · L-1 and (0.45 ± 0. 0 8) ng · L-1, P <0.05; 9) ng · L-1. After 60 min of reperfusion, the level of serum IL-8 was (0.87 ± 0.29) ng · L-1 and (0.47 ± 0.60) ng · L-1 The serum level of IL-8 at 120 min was (1.03 ± 0.43) ng · L-1 and (0.44 ± 0.0 7) ng · L-1, respectively, all at P reperfusion 1, the abnormal ultrastructure changes; the concentration of IL-8 at different time points in XNJI group was significantly lower than that in IR group (0.48 ± 0.0 7 ng · L-1 at 30 min after XNJI group, P <0 .0 5; reperfusion 30min was (0.05 ± 0.08) ng · L-1; reperfusion 60min was (0.55 ± 0.14) ng · L-1; reperfusion 12 0min (0. 59 ± 0.17) ng · L-1, P <0.01 after reperfusion at different time points).