1介绍发现新的抗癌药物的研究目前正面临着重要的挑战,因为在临床前研究显示有效果的化合物只有5%的能继续被开发,成为获得许可的药物。传统的2D细胞培养模型在药物发现过程的早期阶段被用于评估候选药物,然而,有越来越多的证据表明,在二维单层生长的细胞并不能准确地反映肿瘤的生物学复杂性。对兼容高通量筛选的更好的体外模型的需求 1 Introduction The discovery of new anti-cancer drugs is currently facing significant challenges as only 5% of the compounds that have been shown to be effective in the preclinical studies continue to be developed and become licensed drugs. Traditional 2D cell culture models have been used to evaluate drug candidates early in the drug discovery process. However, there is growing evidence that cells grown in 2D monolayers do not accurately reflect the biological complexity of the tumor . The need for a better in vitro model compatible with high-throughput screening