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目的检测三阴乳腺癌(TNBC)组织中C-myc和Skp2的表达情况及其意义。方法选取2008年1月-2011年12月手术切除的TNBC标本56例,采用免疫组织化学链霉素抗生物素蛋白-过氧化物酶连结法检测它们和30例癌旁正常乳腺组织中C-myc和Skp2的表达情况。结果 TNBC组织中C-myc和Skp2表达定位于细胞核,其表达率分别为57.14%(32/56)和58.93%(33/56),明显高于癌旁正常组织(P<0.05);56例TNBC组织中,C-myc和Skp2蛋白在组织学分级Ⅲ级表达率高于Ⅰ+Ⅱ级(P<0.05);C-myc和Skp2的病理分期Ⅲ期的表达率高于Ⅰ-Ⅱ期(P<0.05);表达率高低与腋窝有无淋巴结转移有关(P<0.05),而与病理组织类型无关(P>0.05);相关性分析显示,C-myc和Skp2二者之间呈正相关(P<0.05)。结论 C-myc和Skp2在TNBC中呈明显高表达,与TNBC的发生、发展和转移密切相关。
Objective To detect the expression of C-myc and Skp2 in three negative breast cancer (TNBC) tissues and their significance. Methods Fifty-six TNBC specimens surgically removed from January 2008 to December 2011 were selected. Immunohistochemical streptavidin-peroxidase-linked immunosorbent assay (ELISA) was used to detect the expression of C- myc and Skp2 expression. Results The expressions of C-myc and Skp2 in the nucleus of TNBC were located in the nucleus. The expression rates of C-myc and Skp2 were 57.14% (32/56) and 58.93% (33/56) respectively, which were significantly higher than those in normal tissues (P <0.05) In TNBC tissues, the expression rates of C-myc and Skp2 in histological grade Ⅲ were higher than those in Ⅰ + Ⅱ (P <0.05); the expression rates of C-myc and Skp2 in stage Ⅲ were higher than those in stage Ⅰ-Ⅱ P <0.05). The expression level was correlated with axillary lymph node metastasis (P <0.05), but not with pathological type (P> 0.05). Correlation analysis showed that there was a positive correlation between C-myc and Skp2 P <0.05). Conclusion C-myc and Skp2 are highly expressed in TNBC, which is closely related to the occurrence, development and metastasis of TNBC.