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弓形体病是一种人畜共患传染病,我们于1983年10月对小松定高养猪场进行了初步调查。猪血清采自定高养猪场,均经56℃30分钟灭活,再用2%小牛血清盐水稀释。如血清有正常血凝素,应先用正常绵羊红细胞吸收后再稀样。抗原致敏红细胞系省流行病研究所制备。间接血凝试验用微量法。被检血清1:32~1:128稀释,以1:64(++)以上为阳性。结果:采用间接血球凝集试验(HA)检查102头外表健康猪的血清,其中有6头呈阳性反应,阳性率为5.9%,从而表明这些猪曾感染过弓形体,提示猪弓形体感染在我县存在。 (参加此项工作的尚有:省流研所薛在椿,建瓯县卫生防疫站张琍玲,建瓯县畜牧兽医站张国华)
Toxoplasmosis is a zoonotic infectious disease, and we conducted a preliminary survey of Komatsuding Hogwash in October 1983. Pig serum collected from high pig farms were inactivated at 56 ℃ for 30 minutes and then diluted with 2% fetal bovine serum. If the serum has normal hemagglutinin, it should be absorbed by normal sheep erythrocytes and then diluted. Antigen-primed erythrocytes were prepared by the Provincial Institute of Epidemiology. Indirect hemagglutination test with trace method. Serum was seized 1: 32 ~ 1: 128 dilution to 1:64 (++) or more positive. Results: Serum samples of 102 healthy pigs were examined by indirect hemagglutination test (HA), of which 6 were positive and the positive rate was 5.9%, indicating that these pigs had been infected with Toxoplasma gondii, suggesting that Toxoplasma gondii infected me County exists. (Participate in this work are still: Provincial Institute of Flow Xue in Chun, Jian’ou Health and Epidemic Prevention Station Zhang Ling, Jianou County Animal Husbandry and Veterinary Station Zhang Guohua)