在牧草的固氮细菌中含脂螺菌(AzospirilI um spp.)是最重要的一种。一般说来是把土壤或根段接种在无氮培养基上,从培养基上分离得到含脂螺菌。然而,Dobereiner等人对于从温带地区收集的样品,从中获得纯养培物是困难的,这一点已做过报道。Vlassk和Reynders已经发现在许多情况下,固氮细菌与其它微生物紧密地联在一起,所以获得纯的 Among the nitrogen-fixing bacteria in pasture, Azospirilum upps is the most important one. In general, soil or root segments are inoculated on a nitrogen-free medium and liposomal spores are isolated from the medium. However, it has been reported that it is difficult for Dobereiner et al. To obtain pure cultures from samples collected from temperate regions. Vlassk and Reynders have found that in many cases nitrogen-fixing bacteria are closely linked to other microbes, so obtaining pure