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目的探讨氨溴索对小鼠放射性肺损伤的保护作用。方法采用雄性BALB c小鼠,体重25g左右,随机分为空白对照组(C)、单纯放射组(SI)和照射加氨溴索组(SI+A),每组80只,观察8个月。采用4MVX线直线加速器照射,射野2cm×2.5cm。常规照射,总吸收剂量为25Gy5次。氨溴索口服吸收50mg·kg-1·d-1,照射前1周服用至实验结束。照射后1～8个月取右肺组织进行羟脯胺酸含量测定,左肺组织用于病理检测,血浆进行转化生长因子β1(TGFβ1)含量测量。结果SI组羟脯胺酸含量从第3个月开始增高,6月时最高,与7和8月基本一致。6月时,SI组明显高于SI+A组(P<0.01);SI+A组明显高于C组(P<0.01);TGFβ1含量从第2个月开始上升,3月达到高峰,4、5月开始下降,持续到8月。3个月时,SI组明显高于SI+A组(P<0.01);SI+A组高于C组(P<0.01)。结论氨溴索通过羟脯胺酸和TGFβ1含量的改变减轻放射性肺损伤。
Objective To investigate the protective effect of ambroxol on radiation-induced lung injury in mice. Methods Male BALB c mice weighing 25 g were randomly divided into blank control group (C), radiation alone group (SI) and radiation plus ambroxol group (SI + A) . Using 4MVX line linear accelerator irradiation, field 2cm × 2.5cm. Routine irradiation, the total absorbed dose of 25Gy5 times. Ambroxol orally absorbed 50mg · kg-1 · d-1, taken 1 week before irradiation to the end of the experiment. The right lung tissue was taken from 1 to 8 months after irradiation to determine the content of hydroxyproline, the left lung tissue was used for pathological examination, and the content of transforming growth factor β1 (TGFβ1) in plasma was measured. Results The content of hydroxyproline in SI group increased from the third month to the highest in June and was consistent with that in July and August. At 6 months, SI group was significantly higher than SI + A group (P <0.01); SI + A group was significantly higher than C group (P <0.01); TGFβ1 content increased from the second month, , May began to decline, continuing until August. At 3 months, SI group was significantly higher than SI + A group (P <0.01); SI + A group was higher than C group (P <0.01). Conclusion Ambroxol can reduce radiation-induced lung injury through the changes of hydroxyproline and TGFβ1.