含人反意基因的假型逆转录病毒已在许多实验室构建,并用于抑制病毒的或细胞的基因表达。抑制基因活性是一个新的遗传分析方法,其机制是通过一条互补于正常RNA的反意链与有意义链的结合而影响其功能,它的优点是能根据目的基因的转录产物设制有效的反意RNA,研究该基因在细胞中的生物学作用。RNA逆转录病毒因其独特性结构和繁衍形式,已成为理想的基因转移载体。含人 Pseudotyped retroviruses containing human antisense genes have been constructed in many laboratories and used to inhibit viral or cellular gene expression. Inhibiting gene activity is a new genetic analysis method. Its mechanism is to influence its function through the combination of an anti-sense and a sense strand that is complementary to normal RNA. Its advantage is that it can be effectively designed according to the transcript of the target gene. Antisense RNA to study the biological role of this gene in cells. RNA retrovirus has become an ideal gene transfer vector because of its unique structure and propagation pattern. People included