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为明确日本看麦娘抗性种群对精噁唑禾草灵的抗性水平及抗性产生的分子机制,采用整株水平测定法测定了日本看麦娘对精噁唑禾草灵的抗性水平,扩增和比对了日本看麦娘抗性和敏感种群间乙酰辅酶A羧化酶(acetyl-Co A carboxylase,ACCase)基因的差异。结果显示,与敏感种群AH-7相比,抗性种群AH-25对精噁唑禾草灵的抗性倍数为33.82;AH-25种群ACCase基因CT区域2 078位氨基酸发生了突变,由天冬氨酸GAT突变为甘氨酸GGT;AH-25种群对炔草酯、烯草酮和烯禾啶产生了高水平的抗性,抗性倍数分别为35.66、38.64和29.14,对高效氟吡甲禾灵产生了低水平的抗性,抗性倍数为3.04,对精喹禾灵和唑啉草酯较敏感。表明ACCase基因2 078位氨基酸的突变可能是导致精噁唑禾草灵产生高水平抗性的重要原因。
In order to clarify the resistance mechanism of Ophiopogon japonicus to fenoxaprop in Japan and the molecular mechanism of resistance, we measured the resistance of Ophiopogon japonicus to fenoxaprop-citrate using whole-plant level assay Level, and amplified and compared the differences of the acetyl-CoA carboxylase (ACCase) gene in Ophiopogon japonicus between resistant and susceptible populations in Japan. The results showed that the resistant ratio of resistant population AH-25 to fenoxaprop-citrate was 33.82 compared with the susceptible population AH-7. The amino acid at position 2,078 of CTase of AH-25 population was mutated from day Aspartate GAT mutated to glycine GGT; AH-25 populations produced high levels of resistance to clodinafop propargyl, clethodim and enoxim, with resistance multiples of 35.66, 38.64 and 29.14, respectively, Spirit produced a low level of resistance with a resistance multiple of 3.04 and was more sensitive to quizalofop and pinoxaden. The results showed that the mutation of amino acid at position 2,078 of ACCase gene may be the important reason for the high level of resistance of fenoxaprop in grasshopper.