[Objective] The aim of this study was to develop a quantitative PCR detection method for genetically modified maize event NK603, so as to provide basis for quantitative analysis of event NK603. [Methods] A quantitative PCR detection method for genetically modified maize event NK603 was developed using primers and Taqman probe designed according to the flanking sequence of event NK603, which was then adopted to detect the samples containing 2% NK603 standard (with uncertain quantity of 10%). [Results] The slope of standard curve ranged between -3.6 and -3.1, and the correlation coefficient was higher than 0.99. The amplification efficiency of this method reached 100.2%, fallen between 90% and 110%. The detected quantity of the experimental sample was 1.9%, closer to the true quantity (2%). [Conclusion] This quantitative PCR detection method for genetically modified maize event NK603 is very precise and can be adopted in routine testing analysis. [Objective] The aim of this study was to develop a quantitative PCR detection method for genetically modified maize event NK603, so as to provide basis for quantitative analysis of event NK603. [Methods] A quantitative PCR detection method for genetically modified maize event NK603 was developed using primers and Taqman probes designed according to the flanking sequence of event NK603, which was then adopted to detect the samples containing 2% NK603 standard (with an uncertainty quantity of 10%). [Results] The slope of standard curve ranged between -3.6 and -3.1, and the correlation coefficient was higher than 0.99. The amplification efficiency of this method reached 100.2%, fallen between 90% and 110%. The measured quantity of the experimental sample was 1.9%, closer to the true quantity (2% ). This quantitative PCR detection method for genetically modified maize event NK603 is very precise and can be adopted in routine testing analysis.