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目的 :研究大鼠慢性饮酒过程中胃粘膜的细胞凋亡和细胞增殖变化的规律 ,探讨慢性酒精刺激对胃粘膜的自我保护作用的影响。方法 :以 6% (V/V)的酒精作为大鼠饮水的唯一来源 ,利用流式细胞仪定量分析大鼠饮酒的不同时程内胃粘膜细胞的细胞周期和细胞凋亡 ,同时用免疫组化方法定性观察了大鼠胃粘膜的细胞增殖和凋亡情况。结果 :饮酒 3～ 2 8天的大鼠胃粘膜的细胞凋亡较对照组明显增加 ,在饮酒 3～ 14天的大鼠胃粘膜其细胞增殖也明显增加 ,提示在此时期内胃粘膜的细胞更新增强。结论 :长期低浓度酒精反复刺激可以促进胃粘膜的细胞凋亡并引起大鼠胃粘膜的自我保护作用减弱
Objective: To study the regularity of gastric mucosal apoptosis and cell proliferation in rats during chronic alcohol consumption and to explore the effect of chronic alcohol stimulation on gastric mucosal self-protection. Methods: 6% (V / V) alcohol was used as the sole source of drinking water in rats. Flow cytometry was used to quantitatively analyze the cell cycle and apoptosis of gastric mucosal cells in drinking different time course. Methods of qualitative observation of rat gastric mucosa cell proliferation and apoptosis. Results: Apoptosis of gastric mucosa in rats after drinking for 3 ~ 28 days was significantly higher than that of the control group, and the proliferation of gastric mucosa in rats was also significantly increased from 3 to 14 days after drinking alcohol, suggesting that during this period, the cells of gastric mucosa Update enhanced. Conclusion: Repeated stimulation with low concentration of alcohol for a long time can promote apoptosis of gastric mucosa and decrease the self-protective effect of gastric mucosa in rats