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本实验在建立DNA多聚酶α活性测定方法的基础上观察了商陆多糖I(PAP-I)对小鼠淋巴细胞增殖及淋巴细胞DNA多聚酶α活性的影响。采用[3H]-TdR掺入法和模板活化法([3H]-dTTP掺入法)分别检测PAP-I给药组及对照组小鼠淋巴细胞增殖能力和淋巴细胞DNA多聚酶α活性。体外实验发现PAP-I显著增强ConA诱导的小鼠脾淋巴细胞增殖及其DNA多聚酶α的活性;小鼠腹腔注射PAP-I(每周1次)对脾淋巴细胞DNA多聚酶α活性基本上无影响,但加入ConA(5μg/ml)刺激后,PAP-I10mg/kg剂量组DNA多聚酶α活性显著增强。结果提示PAP-I增强DNA多聚酶α活性可能是促进脾淋巴细胞增殖、增强免疫功能的机制之一。
In this experiment, based on the establishment of DNA polymerase α activity assay method, the effects of PAP-I on lymphocyte proliferation and lymphocyte DNA polymerase α activity in mice were observed. The [3H]-TdR incorporation method and the template activation method ([3H]-dTTP incorporation method) were used to detect the lymphocyte proliferation and lymphocyte DNA polymerase alpha activity in PAP-I-treated and control mice. In vitro experiments found that PAP-I significantly enhanced ConA-induced mouse spleen lymphocyte proliferation and DNA polymerase alpha activity; mice injected intraperitoneally with PAP-I (once per week) had no effect on spleen lymphocyte DNA polymerase alpha activity However, after stimulation with ConA (5 μg/ml), the activity of DNA polymerase α was significantly increased in the PAP-I 10 mg/kg dose group. The results suggest that PAP-I enhances DNA polymerase alpha activity may be one of the mechanisms that promote spleen lymphocyte proliferation and enhance immune function.