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Objective:To investigate the significance of Poly(ADP-ribose)glycolhydrolase(PARG)and membrane type 1matrix metalloproteinase(MT1-MMP)expressions in human colorectal carcinoma.Methods:Immunohistochemical staining for PARG and MT1-MMP was carried out on colorectal adenoma-carcinoma tissue microarrays containing normal colorectal mucosae,adenoma,adenoma with malignant transformation and adenocarcinoma(total 130 specimens).The expressions of PARG and MT1-MMP in the GLTN[Gallotannin]-treated and GLTN-untreated lovo cells were detected by West Blot.Results:PARG expression in adenocarcinoma(83.1%)and adenoma with malignant transformation(66.7%)was significantly higher than that in normal colorectal mucosa(10%)and adenoma(10.5%).Expression of MT1-MMp in normal colorectal mucosa and adenoma was negative,while the expression in adenocarcinoma(80.3%)and adenoma with malignant transformation(72.2%)was high.The expressions of PARG and MT1-MMP in adenocarcinoma with metastasis and in late tumor stages were significantly higher than those in adenocarcinoma with no metastasis and in early tumor stages.Thus,PARG expression shows a positive correlation with the expression of MT1-MMP.The expressions of PARG and MT1-MMP in GLTN-treated lovo cells were weaker than that in GLTN-untreated lovo cells.Conclusion:The expression of PARG was probably related to the development of colorectal carcinoma.PARG may play an important role for the regulation of MT1-MMP expression in colorectal carcinoma.