目的　探讨宿主细胞表达的人血管内皮抑制素 (endostatin)对人肝癌细胞在体内生长的影响。　方法　利用逆转录病毒载体pLncx,将人endostatin基因导入人肝癌细胞SMMC772 1内建立转基因肝癌细胞株。应用PCR、免疫组化及Westernblot检测人endostatin的转染、表达和分泌。血管内皮细胞增殖试验检测表达的endostatin生物学活性。同时对转染细胞株在体外及在裸鼠体内的生长情况进行观察。　结果　PCR证实 ,转endostatin基因的肝癌细胞基因组中存在有 550bp人特异性endostatin片段 ,免疫组化及Westernblot印迹分析示人endostatin在转染肝癌细胞株中获得稳定表达和分泌。转染细胞表达的endostatin能显著抑制人血管内皮生长 ,抑制率达 48% (P <0 0 1 )。与对照组相比 ,转染人endostatin基因的肝癌细胞在体外的生长速度无明显改变 ,但在接种裸鼠皮下后 ,肿瘤生长明显受到抑制 ,在 2 2d时 ,肿瘤缩小率达 94 5 % (P <0 0 1 )。　结论　逆转录病毒介导的人endostatin基因疗法对人肝癌SMMC772 1在裸鼠体内的生长有显著抑制作用 Objective To investigate the effect of human endostatin expressed on host cells on the growth of human hepatoma cells in vivo. Methods The human endostatin gene was introduced into human hepatocellular carcinoma cell line SMMC772 1 using retroviral vector pLncx to establish a transgenic hepatoma cell line. The transfection, expression and secretion of human endostatin were detected by PCR, immunohistochemistry and Western blot. Endothelial cell proliferation assay detects the expression of endostatin biological activity. Meanwhile, the growth of transfected cell lines in vitro and in nude mice were observed. Results PCR confirmed that there was a 550bp human endostatin fragment in the endostatin gene-transfected liver cancer cell lines. Immunohistochemistry and Western blot analysis showed that human endostatin was stably expressed and secreted in the transfected hepatoma cells. Endostatin transfected cells could significantly inhibit the growth of human vascular endothelial cells, the inhibition rate was 48% (P <0.01). Compared with the control group, the growth of hepatocarcinoma cells transfected with human endostatin gene did not change significantly in vitro, but the tumor growth was significantly inhibited after inoculation of nude mice subcutaneously. At 22 days, the tumor shrinkage rate reached 94.5% P <0 0 1). Conclusions Retrovirus-mediated human endostatin gene therapy significantly inhibits the growth of human hepatoma SMMC772 1 in nude mice