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人芽囊原虫(Blastocystis hominis,Bh)是一种可引起腹泻的病原体,已受到国内外医务工作者的重视.多年来,我们对临床疑为肠道原虫感染的急慢性腹泻患者的粪便标本及在医学生中进行调查;并对确诊病例随访观察.现将实验结果总结如下.材料与方法一、材料来源(一)收集广州市各医院门诊疑为肠道原虫感染的急慢性腹泻患者的粪便标本.(二)随机收集我校1994、1995和1996年级本科医学生、部分研究生和专科班学生的粪便标本.二、实验方法(一)采用生理盐水直接涂片法和碘液染色法进行活体标本观察:查出人芽囊原虫病原体后固定涂片,采用肠道原虫三色染色法和铁苏木素染色法染色;用AO显微镜在高倍和油镜下作虫种鉴定;Bh阳性者给于抗寄生虫及抗真菌类药物治疗.
Blastocystis hominis (Bh) is a causative agent of diarrhea and has been valued by medical staff both at home and abroad.For many years, we have evaluated the stool specimens of patients with acute and chronic diarrhea who are suspected of enterovirus infection In medical students to investigate and follow-up observation of the confirmed cases.Experimental results are summarized as follows.Materials and methods First, the source of the material (A) to collect the Guangzhou City, the hospital outpatient suspected intestinal protozoal infection in patients with acute and chronic diarrhea stool Specimens (2) Stool samples of undergraduates, some graduate students and junior college students from 1994, 1995 and 1996 in our school were randomly collected. (2) Experimental methods (1) The specimens were stained with saline and iodine solution Specimen observation: After identification of the pathogen of Blastocystis protozoa, the smear was fixed by trichrome staining of intestinal protozoa and stained with iron hematoxylin; AO was used for identification of worms under high magnification and oil-immersing microscope; Parasites and antifungal agents.