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目的研究C/EBPβ在先天性马蹄内翻足患者足踝部肌肉组织中的表达,探讨细胞系的选择。方法应用RT-PCR、免疫组化、免疫荧光等技术分别检测先天性马蹄内翻足患者足踝部肌肉组织中C/EBPβ的表达及相应的形态学改变。结果与同龄对照相比,C/EBPβ在先天性马蹄内翻足患者足踝部肌肉组织中表达明显下调,相对于正常组织,病变组织结构紊乱,核脱失。免疫荧光结果提示C/EBPβ主要在细胞质中表达,但在肿瘤细胞如人横纹肌肉瘤细胞中呈现细胞质、细胞核均一性表达。结论 C/EBPβ的低表达可能与先天性马蹄内翻足畸形发生相关,病变组织细胞核可能已经失去功能,可能存在纤维化。
Objective To investigate the expression of C / EBPβ in muscle tissue of ankle and foot of patients with congenital clubfoot and to explore the choice of cell lines. Methods RT-PCR, immunohistochemistry and immunofluorescence were used to detect the expression of C / EBPβ and its corresponding morphological changes in the ankle and foot muscles of patients with congenital clubfoot. Results Compared with the controls of the same age, C / EBPβ expression was significantly down-regulated in the foot and ankle muscles of patients with congenital clubfoot. Compared with normal tissues, the lesions were disorganized and the nuclei were lost. The results of immunofluorescence indicated that C / EBPβis mainly expressed in the cytoplasm, but cytoplasm appears in tumor cells such as human rhabdomyosarcoma cells, and the nucleus is uniformly expressed. Conclusion The low expression of C / EBPβ may be related to the congenital clubfoot deformity. The diseased tissue nucleus may have lost function and fibrosis may exist.