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[目的]探讨聚合酶链反应(PCR,Polyerase Chain Reaction)在肺结核诊断中的临床应用研究价值。[方法]对116例肺结核及肺部其他疾病患者进行痰PCR和涂片结核杆菌的检查以及PPD皮试法,其中88例确诊为肺结核患者被作为结核病组,同时另外选取同期接治的48例非结核患者作为比对为对照组,将两组间痰PCR法与涂片法及PPD皮试法的结果比较。[结果]患者痰结核杆菌的PCR检出阳性率为70.5%,明显高于涂片法的检出率35.2%和PPD皮试法的检出率25.0%。[结论]PCR检测技术临床上对肺结核的诊断和鉴别具有较好的应用价值。
[Objective] To investigate the clinical value of polymerase chain reaction (PCR) in the diagnosis of pulmonary tuberculosis. [Method] The sputum PCR and smear Mycobacterium tuberculosis were detected in 116 patients with pulmonary tuberculosis and other pulmonary diseases, and 88 patients with tuberculosis diagnosed as tuberculosis were selected as experimental group and another 48 patients Non-tuberculosis patients as a comparison for the control group, the sputum PCR between the two groups smear method and PPD skin test results comparison. [Results] The positive rate of PCR detection for sputum Mycobacterium tuberculosis was 70.5%, which was significantly higher than that of smear method 35.2% and PPD skin test method 25.0%. [Conclusion] PCR detection technology has a good clinical value in the diagnosis and differential diagnosis of pulmonary tuberculosis.