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目的:探索用于蛋白质定量分析增敏效果的方法。方法:研究发现在1.0 mol/L的硫酸介质中有十二烷基磺酸钠(SDS),蛋白质与钙指示剂羧酸钠作用形成复合物,使最大波长350 nm的共振光散射光谱得到加强,根据其共振光散射的增强程度,可定量测定蛋白质,SDS的加入,使灵敏度提高3.5倍。结果:在选定条件下,几种蛋白质在0.008~25μg/m l浓度范围内与ΔI350nm呈线性关系,建立了定量测定蛋白质的新方法。结论:该法操作简便,灵敏度高,线性范围宽,重现性较好,用于人血清中总蛋白质的测定,结果与经典的考马斯亮蓝法一致。
Objective: To explore a method for the quantitative analysis of protein sensitizing effect. Methods: Sodium dodecyl sulfate (SDS) was found in 1.0 mol / L sulfuric acid medium. The complex formed by the interaction between calcium and sodium carboxylate, enhanced the resonance light scattering spectrum with the maximum wavelength of 350 nm , According to its enhanced degree of resonance light scattering, quantitative determination of protein, SDS, so that the sensitivity increased by 3.5 times. Results: Under the selected conditions, several proteins showed a linear relationship with ΔI350nm in the concentration range of 0.008 ~ 25μg / ml, and a new method for the quantitative determination of protein was established. Conclusion: The method is simple, sensitive, broad linear range, reproducible, and is suitable for the determination of total protein in human serum. The results are consistent with the classical Coomassie brilliant blue method.