论文部分内容阅读
目的通过观察鱼藤酮处理大鼠脑内相关脑区5-羟色胺(5-HT)和5-羟色胺转运体(SERT)的表达变化,探讨鱼藤酮对5-HT能神经元的影响。方法选用健康成年雄性Wistar大鼠42只,背部皮下注射鱼藤酮制作帕金森病大鼠动物模型,以免疫组织化学染色、免疫印迹法及流式细胞术显示大鼠脑内相关脑区5-HT和SERT的表达变化。结果 1.5-HT:免疫组织化学染色显示,鱼藤酮组大鼠中脑中缝背核5-HT免疫反应阳性神经元数量明显少于对照组(P<0.01),中缝背核和尾壳核5-HT免疫反应强度明显减弱(P<0.01);流式细胞术(间接免疫荧光法)检测显示,鱼藤酮组大鼠5-HT的表达量比对照组明显降低(P<0.01)。2.SERT:免疫组织化学染色显示,鱼藤酮组大鼠中缝背核和尾壳核SERT免疫反应强度明显减弱(P<0.01);免疫印迹法检测发现,鱼藤酮组大鼠中缝背核及尾壳核的SERT与β-actin吸光度比值与对照组相比明显降低(P<0.05)。结论鱼藤酮对5-HT能神经元具有明显的神经毒性。
Objective To observe the effect of rotenone on 5-HT neurons by observing the changes of 5-HT and 5-HT (SERT) in brain of rotenone-treated rats. Methods Forty-two healthy adult male Wistar rats were injected subcutaneously with rotenone on their backs to make a rat model of Parkinson’s disease. Immunohistochemistry, flow cytometry and immunoblotting were used to detect the levels of 5-HT and 5-HT SERT expression changes. Results 1.5-HT: Immunohistochemical staining showed that the number of 5-HT immunoreactive neurons in dorsal raphe nucleus of rotenone group was significantly less than that of control group (P <0.01) (P <0.01). The results of flow cytometry (indirect immunofluorescence assay) showed that the expression of 5-HT in rotenone group was significantly lower than that in control group (P <0.01). SERT: Immunohistochemical staining showed that the intensity of SERT immunoreactivity in dorsal raphe nucleus and caudate putamen decreased significantly in rotenone group (P <0.01). Western blotting showed that the dorsal raphe nucleus and caudate putamen The ratio of SERT and β-actin absorbance was significantly lower than that of the control group (P <0.05). Conclusion Rotenone has obvious neurotoxicity on 5-HT neurons.