论文部分内容阅读
目的 探讨淋巴细胞各亚群比例在溃疡性结肠炎 (UC)发病中的变化及意义。方法 利用双色、三色荧光抗体流式细胞术及细胞内细胞因子检测法对 19例UC肠黏膜及外周血淋巴细胞作表型分析。结果 (1)UC患者外周血及肠黏膜T、B细胞百分比与对照组比较 ,均差异无显著性 ;(2 )外周血CD+4、CD+8T细胞百分比与对照组相比无改变 ,肠黏膜CD+4T细胞百分比高于对照组 ,CD+8T细胞百分比低于对照组 ;(3)外周血及肠黏膜Th1/Th2比值均低于对照组。结论 (1)T细胞各亚群的变化在UC发病中起重要作用 ,对T细胞亚群的进一步研究有助于深入揭示其发病机制 ;(2 )肠黏膜及外周血中Th1/Th2比值均明显下降 ,提示Th2细胞是UC病变中的主要辅助细胞群体 ,UC是一种Th2细胞占优势的炎症。
Objective To investigate the changes of lymphocyte subsets in the pathogenesis of ulcerative colitis (UC) and its significance. Methods The phenotypes of 19 cases of UC intestinal mucosa and peripheral blood lymphocytes were analyzed by two-color, three-color fluorescent antibody flow cytometry and intracellular cytokine assay. Results (1) The percentages of T and B cells in peripheral blood and intestinal mucosa in UC patients were not significantly different from those in control group. (2) The percentages of CD + 4 and CD + 8T cells in peripheral blood were not changed compared with those in control group. The percentage of CD + 4T cells in mucosa was higher than that in control group, while the percentage of CD + 8T cells was lower than that in control group. (3) Th1 / Th2 ratio in peripheral blood and intestinal mucosa was lower than that in control group. Conclusions (1) The changes of T lymphocyte subsets plays an important role in the pathogenesis of UC. Further study of T cell subsets is helpful to reveal its pathogenesis in depth. (2) The ratio of Th1 / Th2 in intestinal mucosa and peripheral blood It is suggested that Th2 cells are the main auxiliary cell population in UC lesions. UC is a kind of inflammation that Th2 cells predominate.