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目的：在体外研究高糖刺激人肾脏间质成纤维细胞时，ＨＧＦ、ＴＧＦ－β的表达以及黄芪延缓糖尿病肾病与其的相关性。方法：将人肾脏间质成纤维细胞分别培养于不同浓度的葡萄糖中，于 ２４ ｈ后用 ＲＴ－ＰＣＲ方法检测ＨＧＦ ｍＲＮＡ的转录水平，继而用不同浓度的 ＨＧＦ刺激细胞来现察 ＴＧＦ－βｍＲＮＡ的表达。随即用黄芪药物血清刺激细胞，观察ＨＧＦ的表达情况。结果：高浓度葡萄糖培养的细胞早期就有ＨＧＦ表达，之后表达逐渐下降，而ＴＧＦ－β在此之后出现表达上升。同时外源性ＨＧＦ可以抑制ＴＧＦ－βｍＲＮＡ的表达。黄芪含药血清可以显著刺激细胞表达ＨＧＦ。结论：高糖刺激后可以诱导ＨＧＦ表达，该表达的上升是一种有益的防御反应。此外，外源性加入ＨＧＦ可以降低ＴＧＦ－β的表达，从而促进细胞外基质的降解。黄芪通过诱导ＨＧＦ的产生，起到抗纤维化的作用，从而延缓糖尿病肾病的进展。
OBJECTIVE: To investigate the expression of HGF, TGF-β and the relationship between Astragalus and diabetic nephropathy in high glucose-induced human renal interstitial fibroblasts. METHODS: Human renal interstitial fibroblasts were cultured in different concentrations of glucose, and the mRNA level of HGF mRNA was detected by RT-PCR after 24 h. Then, cells were stimulated with different concentrations of HGF to observe the expression of TGF-β mRNA. expression. The cells were immediately stimulated with Astragalus drug serum and the expression of HGF was observed. RESULTS: HGF was expressed early in cells incubated with high concentrations of glucose, and then gradually decreased, whereas TGF-beta expression increased thereafter. At the same time, exogenous HGF can inhibit the expression of TGF-β mRNA. Astragalus medicated serum can significantly stimulate the cells to express HGF. CONCLUSION: HGF expression can be induced after high glucose stimulation, and this rise in expression is a beneficial defense response. In addition, exogenously added HGF can reduce the expression of TGF-β, thereby promoting the degradation of extracellular matrix. Astragalus plays an anti-fibrotic effect by inducing the production of HGF, thereby delaying the progression of diabetic nephropathy.