虽然TIL可作为LAK样前体细胞应用于临床,但从实体瘤中分离TIL,操作复杂,又消耗多种酶制剂。我们曾报道,从癌性腹水中分离淋巴细胞,不但方法简便,且体外可以扩增并诱导出具有杀伤活性的LAK样细胞,并应用这样的细胞治疗晚期卵巢癌癌性腹水,取得了较满意的疗效。本文应用晚期肺癌癌性胸水中淋巴细胞作为LAK样效应细胞的前体 Although TIL can be used clinically as a LAK-like precursor cell, isolation of TIL from solid tumors is complex and consumes multiple enzyme preparations. We have reported that isolation of lymphocytes from cancerous ascites is not only a simple method, but also can expand and induce LAK-like cells with killing activity in vitro, and use such cells to treat cancerous ascites in advanced ovarian cancer, and has achieved satisfactory results. Efficacy. This article uses advanced lung cancer as a precursor of LAK-like effector cells in lymphocytes in cancerous pleural fluid.