将鹿蹄草科日本石韦(Pyrola japonica)全草(2kg)粉碎,经甲醇回流提取5次,得到甲醇提取物(650g)。甲醇提取物混悬于水中,依次用正己烷、二氯甲烷、乙酸乙酯和正丁醇提取。正丁醇部分上Sephadex LH-20柱,用甲醇-水(0%、30%、50%、70%、100%)洗脱得到14个部分(B-01～14)。各部分分 Pyrola japonica whole plant (2 kg) was pulverized and extracted five times with methanol to give a methanol extract (650 g). The methanol extract was suspended in water and extracted with n-hexane, methylene chloride, ethyl acetate and n-butanol in that order. The n-BuOH fraction was eluted on a Sephadex LH-20 column and eluted with methanol-water (0%, 30%, 50%, 70%, 100%) to obtain 14 fractions (B-01-14). Each part