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目的:研究瘦素和瘦素功能性受体OB-Rb在非小细胞肺癌(non-small cell lung cancer,NSCLC)中的表达,并探讨瘦素和OB-Rb在NSCLC发生、发展过程中的意义。方法:采用RT-PCR和免疫组织化学方法,检测38例NSCLC肿瘤组织及其癌旁组织中瘦素和瘦素受体OB-Rb的mRNA和蛋白表达情况,同时将其蛋白表达水平与NSCLC的临床病理参数进行相关性分析。结果:瘦素与OB-Rb的mRNA在肺癌组织表达水平显著高于癌旁组织(P=0.000)。瘦素蛋白在肺癌组织和癌旁组织的阳性表达率分别为71.1%和26.3%(P<0.005),OB-Rb蛋白在肺癌组织和癌旁组织的阳性表达率分别为63.2%和31.6%(P<0.010);瘦素和OB-Rb蛋白与NSCLC细胞类型、分化程度、TNM分期及淋巴结转移无明显相关性(P>0.05),瘦素与OB-Rb蛋白在NSCLC中的表达也无显著关联(P>0.05)。结论:瘦素和OB-Rb在肺癌组织表达上调,瘦素可能是NSCLC发生、发展过程中的一种促肿瘤生长因子。瘦素和OB-Rb在NSCLC的促癌作用没有直接的协同效应,可能是通过各自不同的机制参与NSCLC的病程。
OBJECTIVE: To study the expression of OB-Rb, a receptor for leptin and leptin, in non-small cell lung cancer (NSCLC) and to investigate the role of leptin and OB-Rb in the development and progression of NSCLC significance. Methods: The mRNA and protein expression of leptin and leptin receptor OB-Rb were detected by RT-PCR and immunohistochemistry in 38 NSCLC tissues and their adjacent tissues. Meanwhile, Clinical pathological parameters were analyzed. Results: The mRNA expression levels of leptin and OB-Rb in lung cancer tissues were significantly higher than those in adjacent tissues (P = 0.000). The positive rates of leptin in lung cancer tissues and paracancer tissues were 71.1% and 26.3%, respectively (P <0.005). The positive expression rates of OB-Rb protein in lung cancer tissues and paracancerous tissues were 63.2% and 31.6% P <0.010). There was no significant correlation between leptin and OB-Rb protein and cell type, differentiation degree, TNM stage and lymph node metastasis (P> 0.05), but there was no significant difference between leptin and OB-Rb protein in NSCLC Correlation (P> 0.05). Conclusion: Leptin and OB-Rb are up-regulated in lung cancer tissues. Leptin may be a tumor-promoting growth factor in the development and progression of NSCLC. There is no direct synergistic effect of leptin and OB-Rb on the carcinogenesis of NSCLC, which may be involved in the course of NSCLC through different mechanisms.