论文部分内容阅读
目的 观察HLA B2 70 4转基因小鼠葡萄膜炎发病情况和糖皮质激素治疗的初步疗效。方法 应用PCR、斑点杂交、Southern杂交、免疫组织化学和流式细胞术进行阳性动物筛选和表达检测。隔日观察眼部症状 ,可疑动物行眼底镜以及裂隙灯检查 ,眼球组织切片行HE染色 ,房水行细胞计数和分类统计。结果 部分HLA B2 70 4转基因阳性鼠出现明显畏光 ,眼科检查发现角膜后沉淀。病理发现角膜上皮细胞增生 ,排列紊乱。房水总细胞数增加 ,炎性细胞与总细胞数之比为9 2 4%。正常对照比值为 0 75 %。 χ2 检验显示二者差异存在显著性 ,P <0 0 5。地塞米松治疗可以减轻症状 ,停药后症状加重。符合前葡萄膜炎诊断。结论 首次在转基因小鼠观察到类似人的HLA B2 7相关性前葡萄膜炎。
Objective To observe the incidence of uveitis in HLA B2 70 4 transgenic mice and the preliminary effect of glucocorticoid therapy. Methods PCR, dot blot hybridization, Southern hybridization, immunohistochemistry and flow cytometry were used to screen and express the positive animals. Eye symptoms were observed every other day, suspicious eyes were examined with ophthalmoscope and slit lamp, HE staining was performed on eye tissue sections, and lineage cell counting and classification statistics were performed. Results Some HLA B2 70 4 transgenic mice showed significant photophobia and ophthalmologic examination revealed post-corneal deposition. Corneal epithelial cells found hyperplasia, arranged disorder. Aqueous total cell number increased, the ratio of inflammatory cells to total cells was 92.4%. The normal control ratio was 0 75%. χ2 test showed significant difference between the two, P <0 05. Dexamethasone treatment can reduce the symptoms, symptoms after stopping withdrawal. In line with the diagnosis of anterior uveitis. Conclusions A similar human HLA B2 7-related anterior uveitis was observed in transgenic mice for the first time.