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目的观察腺苷类似物cyclohexyladenosine(CHA)对大鼠肾小管髓袢升支粗段(medullary thick ascendinglimb,mTAL)管周膜钾离子通道的作用是否通过磷脂酶C-蛋白激酶C途径(phospholipase C-protein kinase C path-way,PLC-PKC pathway)。方法取SD大鼠肾小管髓袢升支粗段放置于倒置显微镜下的浴槽内,利用单通道膜片钳技术观察髓袢升支粗段管周膜钾离子通道活性改变。结果5μmol/L CHA对mTAL管周膜钾离子通道有激活作用(P<0.05)。在加入CHA基础上,分别加入PLC的抑制剂U-73122和PKC的抑制剂Calphostin C,钾离子通道的活性增加(P<0.05)。在加入CHA基础上,加入PKC的激活剂phorbol 12-myristate 13-acetate(PMA),通道活性降低(P<0.05)。结论激活PLC-PKC途径影响CHA对mTAL管周膜50 pS钾离子通道的激活作用。
Objective To observe whether the effect of adenosine analogue cyclohexyladenosine (CHA) on the potassium channels in the peritubular membrane of the medullary thick ascending limb (mTAL) is mediated by the phospholipase C- protein kinase C path-way, PLC-PKC pathway). Methods The tubules of the ascending branch of the renal tubules of SD rats were placed in the bath under the inverted microscope. The changes of the potassium channel activity in the peritumoral membrane of the ascending and descending branches of the medulla oblongata were observed by single-channel patch-clamp technique. Results 5μmol / L CHA could activate the potassium channel of mTAL peritoneal membrane (P <0.05). On the basis of adding CHA, the activity of potassium channel was increased (P <0.05) by adding PLC inhibitor U-73122 and PKC inhibitor Calphostin C respectively. After addition of CHA, the activity of channel was decreased (P <0.05) by adding PKC activator phorbol 12-myristate 13-acetate (PMA). Conclusion Activation of PLC-PKC pathway affects CHA activation of 50 pS potassium channels in mTAL peritumoral membranes.