目的在氯化锂-匹罗卡品诱发癫痫持续状态模型中观察c-fos蛋白表达的时程变化。方法采用氯化锂-匹罗卡品诱发癫痫持续状态大鼠模型,观察大鼠的行为改变,并用免疫组化方法观察癫痫持续状态后不同时间点脑内c-fos蛋白表达的变化。结果癫痫持续状态后6h,1d时脑内c-fos蛋白表达最高,3d时表达次之,7d时极少数表达并接近对照组表达水平。结论癫痫持续状态后脑内c-fos蛋白表达持续较长时间;在此时期应给予干预措施,以减低脑内神经元损伤可能。 Objective To observe the change of c-fos protein expression in the model of status epilepticus induced by lithium-pilocarpine. Methods The rat model of status epilepticus induced by lithium chloride-pilocarpine was induced to observe the behavioral changes in rats. The expression of c-fos protein at different time points after epilepticus status was observed by immunohistochemistry. Results The expression of c-fos protein in brain was the highest at 6h and 1d after status epilepticus. The expression of c-fos protein was the highest at 3d and the lowest at 7d, which was close to that of control group. Conclusion The expression of c-fos protein in the brain persists for a long time after the status epilepticus is senesced. Interventions should be given at this period to reduce the possibility of neuronal damage in the brain.