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目的 探讨强直电刺激大鼠右侧尾壳核 (rightcaudateputamen ,RCPu)重建双侧海马 (hippocampus,HPC)癫痫电网络的细胞机制。 方法 实验共用雄性SD大鼠 10 1只 ,体重 2 0 0～ 2 5 0g。急性强直电刺激 (6 0Hz ,2s,0 .4～ 0 .6mA)RCPu (acutetetanizationoftherightcaudateputamen ,ATRC)或右背HPC(acutetetanizationoftherightdorsalhippocampus,ATRDH) ,同步记录双侧前背HPC神经元单位放电 ,比较激活RCPu或RHPC时 ,神经通路长度对HPC癫痫电网络重建过程中单个神经元电活动的影响。 结果 (1)ATRC和ATRDH均能明显地调制单个HPC神经元的紧张式放电成为节律性爆发式放电。 (2 )ATRC引起的HPC爆发式单位放电串放电时程长[(6 5 0 .738± 5 6 .4 19)ms ,n =12 0 ]、串间隔短 [(interburstinginterval,IBI ,(772 .6 0 0± 4 6 .6 6 5 )ms,n =90 ) ;相反 ,ATRDH引起HPC的爆发式单位放电特征是串放电时程短 [(2 70 .6 12± 19.917)ms,n =12 3](T =6 .35 3,P <0 .0 0 1)、IBI长 [(1373.6 6 3± 12 1.2 36 )ms,n =10 3](T =4 .6 2 7P <0 .0 0 1)。 (3)ATRC诱发的海马细胞单位后放电时程长 [(7.0 6± 0 .776 )s,n =10 4 ]、潜伏期长 [(8.77± 1.2 31)trains ,n =30 ],而ATRDH诱发的单位后放电时程短 [(3.93± 0 .6
Objective To investigate the cellular mechanism of right caudateputamen (RCPu) to reconstruct hippocampus (HPC) epileptic network by tetanic stimulation. Methods A total of 101 male Sprague-Dawley rats weighing 200-250 g were used in the experiment. Acute tetanic electrical stimulation (60 Hz, 2 s, 0.4-0.6 mA) RCPu (acute templatation ofther caudateputamen, ATRC) or right tehperative oftherdorsal hippocampus (ATRDH), simultaneous recording of bilateral front dorsal HPC neurons discharge, more activated RCPu or RHPC, the length of the nerve pathway on the electrical activity of single neurons in the process of HPC epilepsy network reconstruction. Results (1) Both ATRC and ATRDH could obviously modulate the tension discharge of single HPC neurons into rhythmic burst discharge. (2) The length of the discharge of HPC explosive unit discharge caused by ATRC is [(6 5 0 .738 ± 5 6 .4 19) ms, n = 12 0], and the interline interval short [(IBI, (772) In contrast, the explosive discharge characteristic of HPC caused by ATRDH was short time series discharge (2 70.612 ± 19.917) ms, n = 12 3] (T = 6.335, P <0.001), IBI length [(1373.6 6 3 ± 12 1.2 36) ms, n = 10 3] (T = 4 .6 2 7P <0 .0 0 1). (3) ATRC-induced delayed discharge of hippocampal cells [(7.06 ± 0.776) s, n = 104], long latency [8.77 ± 1.231] trains, n = 30] The unit discharge time after short [(3.93 ± 0 .6