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目的:观察丹参注射液对大鼠脊髓损伤(SCI)后低氧诱导因子(HIF)-1α和血管内皮生长因子(VEGF)表达的影响,探讨其参与脊髓损伤修复的机制。方法:选取50只SD大鼠(SPF级)建立Allens’重力打击SCI模型(正常组除外)后,随机分为正常组、模型组、丹参治疗组、丹参加雷帕霉素组和甲泼尼龙琥珀酸钠组(每组10只)。正常组常规饲养,其余实验组均按1 mL·kg~(-1)·d~(-1)剂量分别腹腔注射生理盐水、丹参注射液、丹参注射液(含雷帕霉素3 mg·kg~(-1))和尾静脉注射甲泼尼龙琥珀酸钠溶液30 g·L~(-1)。伤后1,3,7,14 d时采用联合行为评分法(CBS)评价大鼠脊髓神经功能恢复情况。伤后14 d处死动物,采用免疫组化染色和蛋白免疫印迹法(Western-blot)检测各实验组HIF-1α和VEGF的表达差异。结果:SCI后14 d,与正常组比较,模型组大鼠CBS评分升高,HIF-1α和VEGF表达升高(P<005);与模型组比较,丹参治疗组CBS评分降低,HIF-1α和VEGF表达升高(P<005);与丹参治疗组比较,丹参加雷帕霉素组CBS评分升高,而HIF-1α和VEGF表达降低(P<005);上述指标在丹参治疗组和甲泼尼龙琥珀酸钠组之间的差异不具有统计学意义。结论:丹参注射液可通过调节HIF-1α和VEGF的表达从而参与脊髓的损伤修复。
Objective: To observe the effects of Danshen injection on the expression of hypoxia-inducible factor (HIF)-1α and vascular endothelial growth factor (VEGF) after spinal cord injury (SCI) in rats, and to explore the mechanism involved in the repair of spinal cord injury. METHODS: Fifty Sprague-Dawley rats (SPF grade) were established to establish the Allens’ gravity-strike SCI model (except for the normal group) and randomly divided into normal group, model group, Salvia miltiorrhiza treatment group, Dan’s participation in rapamycin group and methylprednisolone. Sodium succinate group (10 in each group). The normal group was fed regularly, and the other experimental groups were given an intraperitoneal injection of physiological saline, Danshen injection, Danshen injection (containing 3 mg·kg of rapamycin) at a dose of 1 mL·kg -1 ·d -1 . ~ (-1)) and tail vein injection of methylprednisolone sodium succinate solution 30 g · L ~ (-1). At 1, 3, 7 and 14 days after injury, the joint function scoring (CBS) was used to evaluate the recovery of spinal nerve function in rats. Animals were sacrificed 14 days after injury. Immunohistochemical staining and Western-blot were used to detect the expression differences of HIF-1α and VEGF in each experimental group. RESULTS: On the 14th day after SCI, compared with the normal group, the CBS score of the model group increased, and the expression of HIF-1α and VEGF increased (P<005). Compared with the model group, the CBS score of the Salvia miltiorrhiza treatment group decreased, HIF-1α The expression of VEGF and VEGF increased (P<005). Compared with the Danshen treatment group, the CBS score of Dan participated in the rapamycin group, while the expression of HIF-1α and VEGF decreased (P<005). The above indicators in the Salvia miltiorrhiza treatment group and The difference between methylprednisolone sodium succinate groups was not statistically significant. Conclusion: Danshen injection can participate in the repair of spinal cord injury by regulating the expression of HIF-1α and VEGF.