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ＫＩ诱导的小麦根液泡膜Ｈ＋－ＡＴＰａｓｅ解离对温度敏感，在４℃时比在２０℃时解离更迅速；该过程为Ｍｇ２＋和ＡＴＰ所加强。其它阴离子诱导酶活性丧失的有效性依次为：ＳＣＮ－＞Ｉ－＞ＮＯ３－＞Ｂｒ－＞Ａｃ－＞ＳＯ３２－＞ＳＯ４２－＞Ｃｌ－＞ＨＣＯ３－。ＳＤＳ－ＰＡＧＥ的结果显示酶活性的丧失伴随着Ｖｏ与Ｖ１的解离。通过透析去除解离剂，可部分恢复泵质子活性和ＡＴＰ水解活性，这表明Ｖｏ与Ｖ１进行重新组装。这种解离和重组的能力在体内Ｖ型Ｈ＋－ＡＴＰａｓｅ的调控和装配上可能有重要意义。
KI induced wheat vacuolar membrane H + -ATPase dissociation is temperature-sensitive and dissociates more rapidly at 4 ° C than at 20 ° C; this process is potentiated by Mg 2+ and ATP. The effectiveness of other anion-induced enzyme activities were as follows: SCN-> I-> NO3-> Br-> Ac-> SO32-> SO42-> Cl-> HCO3-. The results of SDS-PAGE showed that the loss of enzyme activity was accompanied by the dissociation of Vo and V1. Removal of the dissociating agent by dialysis partially restored pump proton activity and ATP hydrolysis activity, indicating that Vo and V1 were reassembled. This ability to dissociate and recombine may be of importance in the regulation and assembly of V-type H + -ATPases in vivo.