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目的研究2008年哈尔滨市手足口病的病原学情况。方法应用RT-PCR和病毒分离两种方法对疑似手足口病患者标本进行检测。结果 RT-PCR快速检测EV71阳性率为25.64%,CA16阳性率0.64%;病毒分离培养EV71阳性率为37.82%,CA16阳性率为2.56%。156份标本分离出阳性病毒株63份,其中EV71阳性59份(占总阳性数的93.65%),CA16阳性4份(占总阳性数的6.35%)。结论哈尔滨市2008年手足口病的流行株为肠道病毒EV71型。手足口病病毒分离培养法阳性率高于RT-PCR法。粪便(包括肛拭子)和咽拭子标本手足口病病毒分离阳性率较高。
Objective To study the etiology of hand, foot and mouth disease in Harbin in 2008. Methods Two methods of RT-PCR and virus isolation were used to detect the specimens suspected of hand-foot-mouth disease. Results RT-PCR showed that the positive rate of EV71 was 25.64% and the positive rate of CA16 was 0.64%. The positive rate of virus isolation and culture was 37.82% and the positive rate of CA16 was 2.56%. Sixty-six positive samples were isolated from 156 samples, of which 59 were positive for EV71 (93.65% of total positive numbers) and 4 were positive for CA16 (6.35% of total positive numbers). Conclusion The epidemic strain of HFMD in Harbin in 2008 was EV71. Hand-foot-mouth disease virus isolation culture method positive rate higher than RT-PCR method. Faeces (including anal swabs) and throat swab specimens hand, foot and mouth disease virus isolation higher positive rate.