自1890年Behring发现抗体以来,免疫学家们曾经为获得单特异的抗体做过许多努力,均未取得成功。直到1975年Kohler和Milstain才成功地将经过绵羊红细胞(抗原)免疫的并已产生抗体的小鼠脾细胞与小鼠骨髓瘤细胞在融合剂──仙台病毒的作用下融合。以后再经筛选和分离纯系,得到了兼 Since the discovery of antibodies by Behring in 1890, immunologists have done a great deal of work to obtain monospecific antibodies and have not succeeded. It was not until 1975 that Kohler and Milstain succeeded in the fusion of sheep erythrocyte (antigen) immunized and antibody-producing mouse splenocytes with mouse myeloma cells under the action of the fusion, Sendai virus. After screening and separation of pure lines, has been and