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【目的】了解病程相关(pathogenesis-related,PR)蛋白质在植物防卫体系中的表达模式进而探讨水稻抗病的分子机理。【方法】选取10个前期工作中鉴定的差异转录PR基因,利用免疫印迹(Western blotting,WB)技术检测它们在水稻正常生长和与白叶枯病菌(Xanthomonas oryzae pv.oryzae Xoo)互作过程中的表达丰度变化。【结果】发现随着水稻的生长,Os12g43380、Os12g36830、Os12g36860、Os12g36840、Os02g41670、Os05g35290和Os12g33610的表达逐步增加,一般在开花期达到最高,成熟期有所降低。在白叶枯病抗性基因Xa21介导的水稻-Xoo非亲和互作过程中,检测到Os01g51570、Os01g71680和Os12g36850的表达量上调,Os12g36830、Os12g36840、Os02g41670和Os05g35290的表达量下调,并且发现,在亲和互作和非亲和互作反应中PR蛋白质的变化模式相似。对PR基因上游启动子区进行分析,发现存在与抗病相关的顺式作用元件,其中,ARE、HSE、MBS、TC-rich repeats等元件在抗病相关的PR基因上游出现频率较高。【结论】鉴定了在水稻-Xoo互作过程中发生丰度变化的7个PR蛋白质。
【Objective】 To understand the expression pattern of pathogenesis-related (PR) proteins in plant defense system and to explore the molecular mechanism of disease resistance in rice. 【Method】 The 10 differentially expressed PR genes identified in the previous work were selected and their expression patterns were examined by Western blotting (WB) in the process of normal rice growth and interaction with Xanthomonas oryzae pv. Oryzae Xoo The expression abundance changes. 【Result】 The results showed that with the growth of rice, the expression of Os12g43380, Os12g36830, Os12g36860, Os12g36840, Os02g41670, Os05g35290 and Os12g33610 gradually increased, and reached the highest in flowering stage and decreased in mature stage. Os01g51570, Os01g71680 and Os12g36850 were up-regulated during the Xoo-Xoo non-affinity interaction of rice leaf blight resistance gene Xoo21, and the expression levels of Os12g36830, Os12g36840, Os02g41670 and Os05g35290 were down-regulated, The pattern of changes in PR proteins was similar in both affinity and non-affinity interactions. The upstream promoter region of PR gene was analyzed and found that there are cis-acting elements related to disease resistance. Among them, elements such as ARE, HSE, MBS and TC-rich repeats appeared higher in the upstream of disease-resistant PR genes. [Conclusion] Seven PR proteins were identified that varied in abundance during the rice-Xoo interaction.